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Registros recuperados: 316 | |
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Ormeño,Juan; Sepúlveda,Paulina; Rojas,Ricardo; Araya,Jaime E. |
Plant samples of jimsonweed (Datura stramonium L.) and thornapple (D. ferox L.) were collected to determine the presence of Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Potato virus Y (PVY) in Santiago, Chile (33º34 S lat, 70º38 W long, altitude 625 mosl), using double stranded RNA (dsRNA) analysis and ELISA. Both weeds were positive to the three types of virus with a percentage of infection ranging from 20-30% except for PVY infection in D. stramonium with an incidence of 5%. Under controlled conditions, the aphid Myzus persicae (Sulzer) transmitted CMV from D. ferox to tomatoes (Lycopersicon esculentum Mill.) and sweet peppers (Capsicum annuum L.), but did not transfer it to potatoes (Solanum tuberosum L.). Seeds of positive D.... |
Tipo: Journal article |
Palavras-chave: AMV; CMV; PVY; Epidemiology. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000400001 |
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Ruiz García, Noé. |
La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta- dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi- cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo una sexta parte del tiempo de procesado. La detectabilidad en impresiones... |
Tipo: Tesis |
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología Citrus tristeza virus; Serology; Sampling; Vectors; Epidemiology. |
Ano: 2012 |
URL: http://hdl.handle.net/10521/1020 |
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Ruiz García, Noé. |
La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta- dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi- cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo una sexta parte del tiempo de procesado. La detectabilidad en impresiones... |
Tipo: Tesis |
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología; Doctorado; Fitopatología; Fitopatología; Serology; Sampling; Vectors; Epidemiology. |
Ano: 2008 |
URL: http://hdl.handle.net/10521/1348 |
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Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.. |
An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to... |
Tipo: Text |
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf |
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Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽. |
DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19... |
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Palavras-chave: Canine; Epidemiology; Feline; Rickettsia; Tick. |
Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/923 |
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Michereff,Sami J; Noronha,Marissônia A; Lima,Gaus SA; Albert,Ígor CL; Melo,Edilaine A; Gusmão,Luciana O. |
The downy mildew, caused by Pseudoperonospora cubensis, is an important melon disease in Northeast Brazil. Considering the lack of standard methods for its assessment, a diagrammatic scale was developed with 2, 4, 8, 16, 32, 64, 82, and 96% of affected leaf area. The scale was then checked for its accuracy, precision, and reproducibility in estimating downy mildew severity. The diagrammatic scale was validated by eight disease raters; using 50 leaves with different severity levels, previously measured using the software Assess®. Two evaluations were performed on the same set of leaves, but in a different sequence order, by the same raters, within a 15-day interval. The accuracy and precision of each rater was determined by simple linear regression between... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis melo; Pseudoperonospora cubensis; Foliar disease; Epidemiology; Phytopatometry. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362009000100015 |
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Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.. |
ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003 |
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Ferreira,Paulo Roberto Abrão; Brandão-Mello,Carlos Eduardo; Estes,Chris; Gonçales Júnior,Fernando Lopes; Coelho,Henrique Sérgio Moraes; Razavi,Homie; Cheinquer,Hugo; Wolff,Fernando Herz; Ferraz,Maria Lúcia Gomes; Pessoa,Mário Guimarães; Mendes-Correa,Maria Cássia. |
Background: Hepatitis C virus infection is a major cause of cirrhosis; hepatocellular carcinoma; and liver transplantation. The aim of this study was to estimate hepatitis C virus disease progression and the burden of disease from a nationwide perspective.Methods: Using a model developed to forecast hepatitis C virus disease progression and the number of cases at each stage of liver disease; hepatitis C virus-infected population and associated disease progression in Brazil were quantified. The impact of two different strategies was compared: higher sustained virological response and treatment eligibility rates (1) or higher diagnosis and treatment rates associated with increased sustained virological response rates (2).Results: The number of infected... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; Disease burden; Epidemiology; Incidence; Brazil. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400363 |
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Cruz Romero, Anabel. |
Los objetivos de esta investigación fueron determinar la distribución espacial e identificar los factores de riesgo asociados a la leptospirosis bovina en el estado de Veracruz, México. Se obtuvieron muestras de sangre de 3,454 bovinos de 54 municipios de las regiones Norte, Centro y Sur del estado, de febrero 2008 a diciembre de 2011. Los sueros se analizaron por medio de la técnica de aglutinación microscópica utilizando 12 serovariedades de Leptospira interrogans, se estimaron las seroprevalencias y los factores de riesgo. La seroprevalencia general fue del 3.9%; sin embargo, entre municipios varió de 0 y 44%. La mayoría de las UP con casos positivos se encontraron en la zona centro de Veracruz, y en los municipios de Cuitláhuac, Papantla, Tlacotalpan... |
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Palavras-chave: Estudio transversal; Bovinos; Serología; Epidemiología; Cross-sectional study; Bovines; Serology; Epidemiology; Agroecosistemas Tropicales; Doctorado. |
Ano: 2013 |
URL: http://hdl.handle.net/10521/2336 |
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DP,Alves; MB,Carneiro; IVF,Martins; CC,Bernardo; DM,Donatele; Pereira Júnior OS,; BR,Almeida; BR,Avelar; AGC,Leão. |
Fasciolosis is a disease that affects the liver parenchyma and bile ducts of numerous animals, including humans, which causes economic losses and threatens public health. The present work aimed at reporting the distribution and factors connected with Fasciola hepatica infection in cattle in ten municipalities in southern Espírito Santo, Brazil, between 2008 and 2009. During this period, 50 rural properties in ten cities were analyzed and fecal samples from 10% of the cattle on each farm were collected. Of the 717 samples analyzed, 154 (21.33%) presented Fasciola hepatica eggs. As for the 50 rural properties studied, 32 (64%) had animals positive for fasciolosis. Throughout the evaluation, it was observed that 42 (84%) rural properties had flooded areas, 33... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fasciolosis; Epidemiology; Ruminants. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300006 |
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Pinto,Tatiana Castro Abreu; Costa,Natália Silva; Souza,Aline Rosa Vianna; Silva,Ligia Guedes da; Corrêa,Ana Beatriz de Almeida; Fernandes,Flavio Gimenis; Oliveira,Ivi Cristina Menezes; Mattos,Marcos Corrêa de; Rosado,Alexandre Soares; Benchetrit,Leslie Claude. |
Streptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agalactiae human and bovine strains isolated between 1980 and 2006 in Brazil. The most prevalent serotypes were Ia, II, III and V and all the strains were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampicin and tetracycline was observed. Among the erythromycin resistant strains, mefA/E, ermA and, mainly, ermB gene were detected, and a shift of prevalence from the macrolide resistance phenotype... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus agalactiae; Pulsed field gel electrophoresis; Epidemiology; Serotyping; Resistance profile. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200003 |
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Moss, Jessica; Behringer, Donald; Shields, Jeffrey D.; Baeza, Antonio; Aguilar-perera, Alfonso; Bush, Phillippe G.; Dromer, Clement; Herrera-moreno, Alejandro; Gittens, Lester; Matthews, Thomas R.; Mccord, Michael R.; Schaerer, Michelle T.; Reynal, Lionel; Truelove, Nathanial; Butler, Mark J.. |
The pathogenic virus Panulirus argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We collected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence was lowest in the southwestern Caribbean. DNA sequence analysis was performed on a fragment of the viral DNA to examine the genetic... |
Tipo: Text |
Palavras-chave: Spiny lobster; Disease; Epidemiology; Connectivity. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25503/23698.pdf |
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Registros recuperados: 316 | |
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